中文Porcine denda remanyoka utachiona RT-PCR yekuona kit
Product Name
Porcine epidemic diarrhea virus RT-PCR Detection Kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Iyi kit inoshandisa chaiyo-nguva fluorescent RT-PCR nzira yekuona iyo RNA yePorcine epidemic diarrhea virus (PEDV) muzvinhu zvechirwere chenyama senge tonsils, lymph nodes uye spleen uye zvinhu zvechirwere chemvura zvakaita sejekiseni uye ropa renguruve.Yakakodzera kuongororwa, kuongororwa uye epidemiological kuongorora yePorcine denda remanyoka utachiona.Iyo kit ndeye YESE-YAGARA PCR SYSTEM (Lyophilized), iyo ine reverse transcriptase, DNA amplification enzyme, reaction buffer, chaiyo primers uye probes inodiwa kuti fluorescent RT-PCR ionekwe.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PEDV PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine PEDV chaiyo zvidimbu |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengeta & Hupenyu Hwesherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye kudzokororwa kwechando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 48 | 8min | / |
| 2 | 1 | 95 | 2min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | PEDV Positive |
| Undet | PEDV Negative |
| 35 | Mhedzisiro inoshupika, ongororazve* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
