Pseudorabies virus (gB) PCR yekuona kit

Tsananguro Pfupi:

Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo RNA yePseudorabies virus (gB gene) (PRV) muzvinhu zvezvirwere zvakadai sematoni, lymph node uye spleen uye mvura yezvirwere zvinhu zvakadai sejekiseni neropa renguruve.


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Chigadzirwa Chigadzirwa

Pseudorabies hutachiona (gB) PCR Yekuona Kit (Lyophilized)

Saizi

48yedzo / kit, 50test / kit

Inotarisirwa Kushandisa

Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo RNA yePseudorabies virus (gB gene) (PRV) muzvinhu zvezvirwere zvakadai sematoni, lymph node uye spleen uye mvura yezvirwere zvinhu zvakadai sejekiseni neropa renguruve. Iyo inokodzera kubatwa, kuongororwa uye epidemiological kuferefetwa kwePseudorabies virus (gB gene). Iyo kit ndeye YOSE-YAKANYANYA PCR SYSTEM (Lyophilized), iyo iine DNA yekukudziridza enzyme, reaction buffer, chaiwo ma primers uye ma probes anodikanwa kune fluorescent RT-PCR kuona.

Chigadzirwa Mukati

Zvikamu Pasuru kutaurwa Ingredient
PRV PCR Sanganisa 1 × bhodhoro (Lyophilized powder)  50Muedzo dNTPs, MgCl2, Primers, MaPes, Taq DNA polymerase
6 × 0.2ml 8 tsvarakadenga-tambo(Ruzivo) 48Muedzo
Kudzora Kwakanaka 1 * 0.2ml chubhu (lyophilized)  10Miedzo

Plasmid kana Pseudovirus ine PRV (gB) zvidimbu zvidimbu

Kupedza mhinduro 1.5 ml Cryotube 500uL /
Negative Kudzora 1.5 ml Cryotube 200uL 0.9% NaCl

Kuchengetedza & Sirifu Hupenyu

(1) Iyo kit inogona kutakurwa pane tembiricha yemukati.

(2) Hupenyu hwesherufu hune mwedzi gumi nemana pa -20 ℃ uye nemwedzi gumi nemiviri pa2 ℃ ~ 30 ℃.

(3) Tarisa chitaridzi pane kit yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized poda vhezheni reagent inofanirwa kuchengetwa pa -20 ℃ mushure mekunyungudika uye kudzokororwa kwechando -thaw inofanira kunge iri pasi pe4 nguva.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Kushanda Dhayagiramu

a) Bhodhoro vhezheni:

1

b) 8 zvakanaka-bvisa chubhu vhezheni:

2

Pcr Kuwedzeredza

Yakakurudzirwa Setting

Nhanho

Kutenderera

Kupisa (℃)

Nguva

Kubwinya kwechiedza

1

1

95

2min

/

2

40

95

5s

/

60

10s

Unganidza FAM fluorescence

* Ongorora: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.

Kududzira Bvunzo Mhedzisiro

Chiteshi

Dudziro yemhedzisiro

FAM Chiteshi

Ct≤35

PRV (gB) Yakanaka

Undet

PRV (gB) Negative

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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