中文Porcine kubereka uye kufema chirwere hutachiona RT-PCR Detection Kit
Product Name
Porcine kubereka uye kufema chirwere hutachiona RT-PCR yekuona kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Iyi kit inoshandisa chaiyo-nguva fluorescent RT-PCR nzira yekuona iyo RNA yePorcine kubereka uye yekufema syndrome hutachiona nucleic acid yekuona kit (PRRSV) mune tishu chirwere zvinhu zvakaita sematonsils, lymph nodes uye spleen uye yemvura chirwere zvinhu zvakaita sejekiseni uye ropa. yenguruve .Yakakodzera kuongororwa, kuongororwa uye epidemiological kuongorora yePorcine Blue Ear Virus.Iyo kit ndeye ALL-ADY PCR SYSTEM (Lyophilized), iyo ine reverse transcriptase, DNA amplification enzyme, reaction buffer, chaiyo primers uye probes inodiwa kuti furuorescent RT-PCR ionekwe.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PBEV PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine PRRSV chaiyo zvidimbu |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 48 | 8min | / |
| 2 | 1 | 95 | 2min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | Vibrio Parahaemolyticus Positive |
| Undet | Vibrio Parahaemolyticus Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
