Swine fever virus RT-PCR Detection Kit

Tsanangudzo Pfupi

Iyi kit inoshandisa chaiyo-nguva fluorescent RT-PCR nzira yekuona iyo RNA ye Swine fever virus (CSFV) mune tishu chirwere zvinhu zvakaita sematonsils, lymph nodes uye spleen uye mvura yechirwere zvinhu zvakaita sejekiseni uye ropa renguruve.


Product Detail

Product Tags

Product Name

Swine fever virus RT-PCR Detection Kit (Lyophilized)

Size

48tests/kiti, 50tests/kiti

Chinangwa Kushandiswa

Iyi kit inoshandisa chaiyo-nguva fluorescent RT-PCR nzira yekuona iyo RNA ye Swine fever virus (CSFV) mune tishu chirwere zvinhu zvakaita sematonsils, lymph nodes uye spleen uye mvura yechirwere zvinhu zvakaita sejekiseni uye ropa renguruve.Inokodzera kutariswa, kuongororwa uye epidemiological kuongorora kweSwine fever virus.Iyo kit ndeye ALL-READY PCR SYSTEM(Lyophilized), iyo ine DNA amplification enzyme, reverse transcriptase, reaction buffer, maprimers chaiwo uye probes inodiwa pakuona kwefluorescent PCR.

Product Contents

Zvikamu Package tsanangudzo Ingredient
CSFV PCR Mix 1 × bhodhoro (Lyophilized poda)  50Test dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) 48Muedzo
Kudzora Kwakanaka 1 * 0.2 ml chubhu (lyophilized)  10Miedzo

Plasmid ine CSFV chaiyo zvidimbu

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 200uL 0.9%NaCl

Kuchengetedza & Hupenyu hweSherufu

(1) Kiti inogona kutakurwa nekamuri tembiricha.

(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.

(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Operation Dhiagiramu

a) Bhodhoro shanduro:

1

b) 8 zvakanaka-strip chubhu shanduro:

2

Pcr Amplification

RecommendedSetting

Danho Cycle Tembiricha (℃) Nguva Fluorescence channel
1 1 50 8min  
2 1 95 2min  
3 40 95 5s  
60 10s Unganidza FAM fluorescence

*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.

Kuturikira Test Results

Channel

Dudziro yezvabuda

FAM Channel

Ct≤35

CSFV Positive

Undet

CSFV Negative

35

Mhedzisiro inoshupika, ongororazve*

*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.


  • Zvakapfuura:
  • Zvinotevera:

  • Related Products