中文Chirwere chetsoka-ne-muromo RT-PCR Detection Kit
Product Name
Chirwere chetsoka-ne-muromo RT-PCR Detection Kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Iyi kit inoshandisa chaiyo-nguva fluorescent RT-PCR nzira yekuona iyo RNA yetsoka-uye-muromo chirwere (CSFV) mune tishu chirwere zvinhu senge tonsils, lymph nodes uye spleen uye mvura chirwere zvinhu zvakaita sejekiseni uye ropa renguruve.Inokodzera kuongororwa, kuongororwa uye epidemiological kuongorora kwetsoka-ne-muromo chirwere.Iyo kit ndeye ALL-READY PCR SYSTEM(Lyophilized), iyo ine DNA amplification enzyme, reverse transcriptase, reaction buffer, maprimers chaiwo uye probes inodiwa pakuona fluorescent PCR.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| FMD PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid ine FMD chaiyo zvidimbu |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
RecommendedSetting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | FMD Positive |
| Undet | FMD Negative |
| 35 | Mhedzisiro inoshupika, ongororazve* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
