Porcine Circovirus mhando 2 PCR yekuona kit

Tsanangudzo Pfupi

Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo RNA yePorcine circovirus mhando 2 (PCV2) mune tishu chirwere zvinhu senge tonsils, lymph nodes uye spleen uye mvura chirwere zvinhu zvakaita sejekiseni uye ropa re.


Product Detail

Product Tags

Product Name

Porcine circovirus mhando 2 PCR Detection Kit (Lyophilized)

Size

48tests/kiti, 50tests/kiti

Chinangwa Kushandiswa

Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo RNA yePorcine circovirus mhando 2 (PCV2) mune tishu chirwere zvinhu senge tonsils, lymph nodes uye spleen uye mvura chirwere zvinhu zvakaita sejekiseni uye ropa re.Inokodzera kuongororwa, kuongororwa uye epidemiological tsvakurudzo yePorcine circovirus type 2. The kit is an ALL-READY PCR SYSTEM (Lyophilized), iyo ine DNA amplification enzyme, reaction buffer, maprimers chaiwo uye probes inodiwa pakutsvaga PCR yefluorescent. .

Product Contents

Zvikamu Package tsanangudzo Ingredient
PCV2 PCR Mix 1 × bhodhoro (Lyophilized poda)  50Test dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase
6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) 48Muedzo
Kudzora Kwakanaka 1 * 0.2 ml chubhu (lyophilized)  10Miedzo

Plasmid kana Pseudovirus PCV2 ine zvimedu zvakati

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 200uL 0.9%NaCl

Kuchengetedza & Hupenyu hweSherufu

(1) Kiti inogona kutakurwa nekamuri tembiricha.

(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.

(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Operation Dhiagiramu

a) Bhodhoro shanduro:

1

b) 8 zvakanaka-strip chubhu shanduro:

2

Pcr Amplification

Inokurudzirwa Setting

Danho

Cycle

Tembiricha (℃)

Nguva

Fluorescence channel

1

1

95

2min

/

2

40

95

5s

/

60

10s

Unganidza FAM fluorescence

*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.

Kuturikira Test Results

Channel

Dudziro yezvabuda

FAM Channel

Ct≤35

Vibrio Parahaemolyticus Positive

Undet

Vibrio Parahaemolyticus Negative

35

Suspicious resut, retest*

*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.


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