中文E.coli O157:H7 PCR yekuona kit
Product Name
E.coli O157:H7 PCR yekuona kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Escherichia coli O157:H7 (E.coli O157:H7) ibhakitiriya re gram-negative rerudzi rweEnterobacteriaceae, rinoburitsa huwandu hukuru hweVero toxin.Kliniki, inowanzoitika kamwe kamwe nekurwadziwa kwakanyanya mudumbu uye manyoka ane mvura, achiteverwa nemanyoka ehemorrhagic mazuva mashoma gare gare, izvo zvinogona kutungamirira kufivha kana kusapisa muviri, uye kufa muzviitiko zvakanyanya.Kiti iyi inokodzera kuongororwa kwemhando yeEscherichia coli O157: H7 muchikafu, masamples emvura, tsvina, marutsi, mvura inosimudzira mabhakitiriya uye mamwe masampuli uchishandisa musimboti wePCR-nguva chaiyo. Lyophilized), iyo ine DNA amplification enzyme, reaction buffer, chaiyo primers uye probes inodiwa kuti fluorescent PCR ionekwe.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| E.coli O157:H7 PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid ine E.coli O157:H7 zvimedu zvakananga |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengeta & Hupenyu Hwesherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye kudzokororwa kwechando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
RecommendedSetting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Chiratidzo cheFAM fluorescence chiteshi chichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Zvakanaka |
| Undet | E.coli O157:H7 Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
