E.coli O157: H7 PCR yekuona kit

Tsananguro Pfupi:

Escherichia coli O157: H7 (E. coli O157: H7) ibhakitiriya rine gramu-risina kunaka rejenasi inonzi Enterobacteriaceae, inoburitsa huwandu hwakawanda hwehuturu hweVero.


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Chigadzirwa Chigadzirwa

E.coli O157: H7 PCR yekuona kit (Lyophilized)

Saizi

48yedzo / kit, 50test / kit

Inotarisirwa Kushandisa

Escherichia coli O157: H7 (E. coli O157: H7) ibhakitiriya rine gramu-risina kunaka rejenasi inonzi Enterobacteriaceae, inoburitsa huwandu hwakawanda hwehuturu hweVero. Kiriniki, inowanzoitika nekukasira nekurwadziwa kwakanyanya kwemudumbu uye manyoka emvura, ichiteverwa nemanyoka ekubuda ropa mazuva mashoma gare gare, izvo zvinogona kukonzeresa kune fivhiri kana kushaya fivhiri, uye kufa mune zvakakomba zviitiko. Iyi kiti yakakodzera kukosheswa kwehunhu hweEscherichia coli O157: H7 mune chikafu, masampula emvura, mutsvina, kurutsa, bhatiri-rinosimudzira mvura uye mamwe masampuli vachishandisa musimboti weiyo-chaiyo-nguva PCR. Lyophilized), iyo iine DNA yekuwedzera enzyme, reaction buffer, chaiwo ma primers uye ma probes anodikanwa kune fluorescent PCR kuona ..

Chigadzirwa Mukati

Zvikamu Pasuru kutaurwa Ingredient
E.coli O157: H7 PCR Sanganisa 1 × bhodhoro (Lyophilized powder)  50Muedzo dNTPs, MgCl2, Primers, MaProbes, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 tsvarakadenga-tambo(Ruzivo) 48Muedzo
Kudzora Kwakanaka 1 * 0.2ml chubhu (lyophilized)  10Miedzo

Plasmid ine E. coli O157: H7 zvidimbu zvakatarwa

Kupedza mhinduro 1.5 ml Cryotube 500uL /
Negative Kudzora 1.5 ml Cryotube 200uL 0.9% NaCl

Kuchengetedza & Sirifu Hupenyu

(1) Iyo kit inogona kutakurwa pane tembiricha yemukati.

(2) Hupenyu hwesherufu hune mwedzi gumi nemana pa -20 ℃ uye nemwedzi gumi nemiviri pa2 ℃ ~ 30 ℃.

(3) Tarisa chitaridzi pane kit yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized poda vhezheni reagent inofanirwa kuchengetwa pa -20 ℃ mushure mekunyungudika uye kudzokororwa kwechando -thaw inofanira kunge iri pasi pe4 nguva.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Kushanda Dhayagiramu

a) Bhodhoro vhezheni:

1

b) 8 zvakanaka-bvisa chubhu vhezheni:

2

Pcr Kuwedzeredza

Yakurudzirwa Kugadzika

Nhanho     Kutenderera Kupisa (℃)   Nguva Kubwinya kwechiedza
1 1 95 2min  
2 40 95 5s  
60 10s Unganidza FAM fluorescence

* Ongorora: Chiratidzo cheFAM fluorescence chiteshi chichaunganidzwa pa60 ℃.

Kududzira Bvunzo Mhedzisiro

Chiteshi

Dudziro yemhedzisiro

FAM Chiteshi

Ct≤35

E.coli O157: H7 Zvakanaka

Undet

E.coli O157: H7 Negative

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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