中文Africa swine fever virus PCR yekuona kit
Product Name
Africa swine fever virus PCR yekuongorora kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo DNA yeAfrica swine fever virus (ASFV)muzvinhu zvechirwere chematishu zvakaita sematonsi, lymph nodes uye spleen uye zvinhu zvechirwere chemvura zvakaita sejekiseni uye ropa renguruve.Inokodzera kuongororwa, kuongororwa uye epidemiological kuongorora kweAfrica swine fever virus.Iyo kit ndeye ALL-ADY PCR SYSTEM (Lyophilized), iyo ine DNA amplification enzyme, reaction buffer, chaiyo primers uye probes inodiwa kuti fluorescent PCR ionekwe.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| ASFV PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid ine ASFV chaiyo zvidimbu |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
RecommendedSetting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | ASFV Positive |
| Undet | ASFV Negative |
| 35 | Mhedzisiro inoshupika, ongororazve* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
