中文Pseudorabies virus (gB) PCR yekuongorora kit
Product Name
Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Iyi kit inoshandisa chaiyo-nguva fluorescent PCR nzira yekuona iyo RNA yePseudorabies hutachiona (gB gene) (PRV) muzvinhu zvechirwere chenyama senge tonsils, lymph nodes uye spleen uye zvinhu zvechirwere chemvura zvakaita sejekiseni uye ropa renguruve.Inokodzera kuongororwa, kuongororwa uye epidemiological kuongorora kwePseudorabies virus (gB gene).Iyo kit ndeye ALL-ADY PCR SYSTEM (Lyophilized), iyo ine DNA amplification enzyme, reaction buffer, chaiyo primers uye probes inodiwa kuti furuorescent RT-PCR ionekwe.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PRV PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine PRV(gB) zvimedu zvakananga |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | PRV(gB) Zvakanaka |
| Undet | PRV(gB) Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
