Norovirus (GⅠ) RT-PCR Detection Kit

Tsanangudzo Pfupi

Inokodzera kuwonekwa kweNorovirus (GⅠ) muhove, miriwo mibishi nemichero, mvura, tsvina, marutsi uye zvimwe zvimiro.Nucleic acid kudhirowa kunofanirwa kuitwa ne nucleic acid yekubvisa kit kana yakananga pyrolysis nzira zvinoenderana neakasiyana emhando mhando.


Product Detail

Product Tags

Product Name

Norovirus (GⅠ) RT-PCR Detection Kit (Lyophilized)

Size

48tests/kiti, 50tests/kiti

Chinangwa Kushandiswa

Inokodzera kuwonekwa kweNorovirus (GⅠ) muhove, miriwo mibishi nemichero, mvura, tsvina, marutsi uye zvimwe zvimiro.Nucleic acid extraction inofanirwa kuitwa ne nucleic acid extraction kit kana yakananga pyrolysis nzira zvinoenderana nemhando dzakasiyana dzemuenzaniso.Kiti ndeye ALL-READY PCR SYSTEM(Lyophilized), iyo ine DNA amplification enzyme, reverse transcriptase,reaction buffer, maprimers chaiwo. uye probes inodiwa pakuonekwa kwefluorescent PCR.

Product Contents

Zvikamu Package tsanangudzo Ingredient
Norovirus (GⅠ) PCR Mix 1 × bhodhoro (Lyophilized poda)  50Test dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) 48Muedzo
Kudzora Kwakanaka 1 * 0.2 ml chubhu (lyophilized)  10Miedzo

Plasmid ine Norovirus (GⅠ) zvimedu zvakananga

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 200uL 0.9%NaCl

Kuchengetedza & Hupenyu hweSherufu

(1) Kiti inogona kutakurwa nekamuri tembiricha.

(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.

(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Operation Dhiagiramu

a) Bhodhoro shanduro:

1

b) 8 zvakanaka-strip chubhu shanduro:

2

Pcr Amplification

RecommendedSetting

Danho Cycle Tembiricha (℃) Nguva Fluorescence channel
1 1 50 8min  
2 1 95 2min  
3 40 95 5s  
60 10s Unganidza FAM fluorescence

*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.

Kuturikira Test Results

Channel

Dudziro yezvabuda

FAM Channel

Ct≤35

Norovirus (GⅠ) Zvakanaka

Undet

Norovirus (GⅠ) Negative

35

Suspicious resut, retest*

*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.


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