中文Salmonella PCR Detection Kit
Product Name
Salmonella PCR Detection Kit (lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Salmonella ndeyeEnterobacteriaceae uye Gram-negative enterobacteria.Salmonella inowanzoitika pathogen inotakura chikafu uye inomira yekutanga muhuturu hwekudya kwebhakitiriya.Zviratidzo zvikuru zvehuturu hwechikafu hunokonzerwa neSalmonella kusvotwa, kurutsa, kurwadziwa mudumbu, kutemwa nemusoro, kupindwa nechando uye manyoka.Iyi kit inoshandisa musimboti weiyo chaiyo-nguva fluorescent PCR uye inokodzera kuongororwa kwemhando yeSalmonella muchikafu, masampu emvura, tsvina, marutsi, uye nekupfumisa mvura.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine zvimedu zvakati |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Unganidza FAM fluorescence |
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | Salmonella Positive |
| Undet | Salmonella Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
