中文Listeria monocytogenes PCR Detection Kit
Product Name
Listeria monocytogenes PCR Detection Kit
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Listeria monocytogenes i gram-positive microbacterium inogona kukura pakati pe4℃ ne45℃.Icho chimwe chezvipembenene zvikuru zvinotyisidzira hutano hwevanhu muzvokudya zvefiriji.Zviratidzo zvikuru zvehutachiona ndezve septicemia, meningitis uye mononucleosis.Kiti iyi yakakodzera kutariswa kwemhando yeListeria monocytogenes muchikafu, masampuri emvura, tsvina, marutsi, mvura inosimudzira bhakitiriya uye mamwe masampuli uchishandisa musimboti weiyo chaiyo-nguva fluorescence PCR. , iyo ine DNA amplification enzyme, reverse transcriptase, reaction buffer, maprimers chaiwo uye probes inodiwa pakuonekwa kwefluorescent PCR.
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| Listeria monocytogenes PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid ine Listeria monocytogenes chaiyo zvidimbu |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a)
b)
Pcr Amplification
RecommendedSetting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | Listeria monocytogenes Positive |
| Undet | Listeria monocytogenes Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
