Staphylococcus aureus PCR Detection Kit

Tsanangudzo Pfupi

Staphylococcus aureus ndeye genus Staphylococcus uye ibhakitiriya re gram-positive.Icho chinowanikwa chikafu chinotakura pathogenic microorganism inogona kuburitsa enterotoxins uye kukonzera chikafu chepfu.


Product Detail

Product Tags

Product Name

Staphylococcus aureus PCR Detection Kit (lyophilized)

Size

48tests/kiti, 50tests/kiti

Chinangwa Kushandiswa

Staphylococcus aureus ndeye genus Staphylococcus uye ibhakitiriya re gram-positive.Icho chinowanikwa chikafu chinotakura pathogenic microorganism inogona kuburitsa enterotoxins uye kukonzera chikafu chepfu.Iyi kit inoshandisa musimboti weiyo chaiyo-nguva fluorescent PCR uye inokodzera kuongororwa kwemhando yeStaphylococcus aureus muchikafu, masampuli emvura, tsvina, marutsi, hupfumi hwemvura uye mamwe masampuli.

Product Contents

Zvikamu Package tsanangudzo Ingredient
PCR Mix 1 × bhodhoro (Lyophilized poda)  50Test dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase
6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) 48Muedzo
Kudzora Kwakanaka 1 * 0.2 ml chubhu (lyophilized)  10Miedzo

Plasmid kana Pseudovirus ine zvimedu zvakati

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 100uL 0.9%NaCl

Kuchengetedza & Hupenyu hweSherufu

(1) Kiti inogona kutakurwa nekamuri tembiricha.

(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.

(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.

(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.

Zviridzwa

GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.

Operation Dhiagiramu

a) Bhodhoro shanduro:

1

b) 8 zvakanaka-strip chubhu shanduro:

2

Pcr Amplification

Inokurudzirwa Setting

Danho

Cycle

Tembiricha (℃)

Nguva

Fluorescence channel

1

1

95

2min

/

2

40

95

6s

/

60

12s

Unganidza FAM fluorescence

Kuturikira Test Results

Channel

Dudziro yezvabuda

FAMChannel

Ct≤35

Staphylococcus aureus Positive

Undet

Staphylococcus aureus Negative

35<Ct40

Suspicioustangazve,ongororazve*

*Kanathe ongororazve mhedzisiro yeFAM chiteshihaskukosha kweCt ≤40uye inoratidza zvakajairikaSshape amplification curve, mhedzisiro inodudzirwa seyakanaka, kana ikasadaro yakaipa.


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