中文Vibrio parahaemolyticus PCR Detection Kit
Product Name
Vibrio Parahaemolyticus PCR Detection Kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Vibrio Parahemolyticus (inozivikanwawo seHalophile Vibrio Parahemolyticus) iGram-negative polymorphic bacillus kana Vibrio Parahemolyticus.ine kutanga, kurwadziwa mudumbu, kurutsa, manyoka uye chituru chemvura sezviratidzo zvikuru zvekliniki.Iyi kit inoshandisa musimboti wenguva chaiyo yefluorescent. PCR uye inokodzera kucherechedzwa kwehunhu hweVibrio Parahaemolyticus muchikafu, masampu emvura, tsvina, marutsi, uye kupfumisa mvura. Iyo kit ndeye ALL-READY PCR SYSTEM(Lyophilized), iyo ine DNA amplification enzyme, reaction buffer, chaiyo primers. uye probes inodiwa pakuonekwa kwefluorescent RT-PCR.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine zvimedu zvakati |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Unganidza FAM fluorescence |
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | Vibrio Parahaemolyticus Positive |
| Undet | Vibrio Parahaemolyticus Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
