中文Norovirus (GⅠ) RT-PCR Detection Kit
Product Name
Norovirus (GⅠ) RT-PCR Detection Kit (Lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Inokodzera kuwonekwa kweNorovirus (GⅠ) muhove, miriwo mibishi nemichero, mvura, tsvina, marutsi uye zvimwe zvimiro.Nucleic acid extraction inofanirwa kuitwa ne nucleic acid extraction kit kana yakananga pyrolysis nzira zvinoenderana nemhando dzakasiyana dzemuenzaniso.Kiti ndeye ALL-READY PCR SYSTEM(Lyophilized), iyo ine DNA amplification enzyme, reverse transcriptase,reaction buffer, maprimers chaiwo. uye probes inodiwa pakuonekwa kwefluorescent PCR.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| Norovirus (GⅠ) PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid ine Norovirus (GⅠ) zvimedu zvakananga |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
RecommendedSetting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Unganidza FAM fluorescence |
*Cherechedza: Zviratidzo zveFAM fluorescence chiteshi zvichaunganidzwa pa60 ℃.
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAM Channel | |
| Ct≤35 | Norovirus (GⅠ) Zvakanaka |
| Undet | Norovirus (GⅠ) Negative |
| 35 | Suspicious resut, retest* |
*Kana mhedzisiro yekuongororazve yeFAM chiteshi iine Ct kukosha ≤40 uye ichiratidza yakajairika "S" chimiro chekusimudzira curve, mhedzisiro inodudzirwa seyakanaka, zvikasadaro haina kunaka.
