中文Staphylococcus aureus PCR Detection Kit
Product Name
Staphylococcus aureus PCR Detection Kit (lyophilized)
Size
48tests/kiti, 50tests/kiti
Chinangwa Kushandiswa
Staphylococcus aureus ndeye genus Staphylococcus uye ibhakitiriya re gram-positive.Icho chinowanikwa chikafu chinotakura pathogenic microorganism inogona kuburitsa enterotoxins uye kukonzera chikafu chepfu.Iyi kit inoshandisa musimboti weiyo chaiyo-nguva fluorescent PCR uye inokodzera kuongororwa kwemhando yeStaphylococcus aureus muchikafu, masampuli emvura, tsvina, marutsi, hupfumi hwemvura uye mamwe masampuli.
Product Contents
| Zvikamu | Package | tsanangudzo | Ingredient |
| PCR Mix | 1 × bhodhoro (Lyophilized poda) | 50Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zvakanaka-strip chubhu(Lyophilized) | 48Muedzo | ||
| Kudzora Kwakanaka | 1 * 0.2 ml chubhu (lyophilized) | 10Miedzo | Plasmid kana Pseudovirus ine zvimedu zvakati |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Kuchengetedza & Hupenyu hweSherufu
(1) Kiti inogona kutakurwa nekamuri tembiricha.
(2) Hupenyu hwesherufu ndeye 18 mwedzi pa -20 ℃ uye 12 mwedzi pa2 ℃ ~ 30 ℃.
(3)Ona iyo label pakiti yezuva rekugadzira uye zuva rekupera.
(4) Iyo lyophilized powder version reagent inofanira kuchengetwa pa -20 ℃ mushure mekunyunguduka uye inodzokororwa chando -thaw inofanira kuva isingasviki ka4.
Zviridzwa
GENECHECKER UF-150, UF-300 chaiyo-nguva fluorescence PCR chiridzwa.
Operation Dhiagiramu
a) Bhodhoro shanduro:
b) 8 zvakanaka-strip chubhu shanduro:
Pcr Amplification
Inokurudzirwa Setting
| Danho | Cycle | Tembiricha (℃) | Nguva | Fluorescence channel |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Unganidza FAM fluorescence |
Kuturikira Test Results
| Channel | Dudziro yezvabuda |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Positive |
| Undet | Staphylococcus aureus Negative |
| 35<Ct≤40 | Suspicioustangazve,ongororazve* |
*Kanathe ongororazve mhedzisiro yeFAM chiteshihaskukosha kweCt ≤40uye inoratidza zvakajairika“S”shape amplification curve, mhedzisiro inodudzirwa seyakanaka, kana ikasadaro yakaipa.
